Syllabus
Week Lecturer Theme: Introduction to Microbiology. Morphology of bacteria.
Physiology of bacteria. Bacterial genetics.
Interaction of bacteria and the host. Viruses and the Host.
Prions. Normal flora.
Principles of antiinfectious imunity mechanisms. Basic properties of pathogenic fungi.
Basic properties of parasites. Antibiotics and antimicrobial therapy.
Rational antimicrobial therapy. Collecting specimens for microbial diagnostics and basic detection techniques.
Nosocomial (Hospital-acquired infections). Disinfection and Sterilisation.
Seminar (Attendance Compulsory): Antiviral, antifungal and antiparazitic therapy. Practicals (one week,): Microscopy in medical bacteriology, mycology and parasitology.
Native, fixed and stained slide. Staining techniques (Gram.
Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Aerobic and anearobic culture (bacterial culture media, cultures and macroscopic morphology.) Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection.
Antigen detection, Typing. Antibiotic susceptibility tests (diffusion disc tests, E-tests, MIC, MBC-testing).
Yeast culture (Sabouraud) and GT-test. Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot).
Genome detection techniques, hybridisation, PCR. Sample collection techniques and Kits, documentation, transport requirements.
Annotation
Provides basic information on clinical microbiology, virology, and parasitology. Based on the recent classification, reviews the most important bacteria, fungi, and parasites of humans, their features, pathogenesis, diseases.
Reviews microbiological diagnostic techniques, prevention, vaccination, and therapeutic methods. During the practicals, provides the opportunity to test correct sampling, subsequent basic laboratory diagnostic methods, pathogen identification, and antibiotics sensitivity testing, and strategy of ATB therapy.