Syllabus
Introduction to Microbiology. Description of basic properties of bacteria, viruses, pathogenic fungi, and parasites. Basic properties of bacteria (morphology, metabolism, cultivation, bacterial genetics). Virulence mechanisms of bacterial pathogens (fimbriae, capsules, cell wall, intracellular parasitism, chemotaxis. Toxins and toxinoses. Antibiotics, mechanisms of action. Disinfection and sterilization. Antiinfectious imunity, active and passive immunisation. Gram-positive cocci, Gram-negative cocci, anaerobic bacteria. Gram-negative rods. Mycobacteria, Spirochaetes, Mycoplasmas. Sample collection techniques and kits, documentation, transport requirements. Viruses, taxonomy, replication viruses, virus-cell interaction. Pathogenesy of viral infections. DNA viruses, RNA viruses.
Demonstration: Native, fixed and stained slide, bacterial cultures and macroscopic morphology, toxin detection, Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot), sample collection kits.
Annotation
Description of basic properties of bacteria, viruses, pathogenic fungi, and parasites. Principles of antiinfectious imunity mechanisms and immunisation.
The most important human pathogens, their possibilities to invade and harm the host, pathogenesis of selected infectious diseases. Collecting specimens for microbial diagnostics and basic detection techniques.
Antibiotics. Disinfection and sterilisation.
Hospital-acquired infections.