Syllabus
Lectures Microbiology 1 (2nd year, Summer Term) Lectures in 2 hour units 30 hours of lectures/termWeek Lecturer Theme
Kolářová: Introduction to Medical Microbiology, Basic Disciplines. Role in Health Care System. Characteristics of microorganisms- bacteria, viruses, fungi and parasites
Pavlik: Identification Techniques for Microbes
Paetříčková: Morphology and Physiology of Bacteria, Bacterial Genetics
Holada: Disinfection and Sterilisation Technics, Methods of Sterility Control
Pavlik: Interaction of bacteria and the host - Pathogenicity and Virulence,
Adámková: Antimicrobial agents and antimicrobial therapy.
Pavlík: Review of Medical Bacteriology. Gram-positive bacteria
Pavlík: Gram-negative Bacteria, Spirochets and Mycoplasma
Pavlík: Mycobacteria, Chlamydiae, Rickettsiae
Mělková: Viruses. Taxonomy. pathogenesis and intracellular parasitism in the host cell
Mělková: Selected DNA Viruses
Holada Prions and prionic infections
Mělková: Selected RNA Viruses
Skořepová: Medical Mycology
Chánová: Parasitology
Seminars (Attendance Compulsory, within the lectures, indicated in the Education Plan):
Diagnostic Techniques in Medical Microbiology (3 hours)
Rational antimicrobial therapy (3 hours)
Practicals (one week, total 24 hours):Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques ? hybridisation, PCR. Horizontál electrophoresis, real-time PCR.Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test.Sample collection techniques and Kits, documentation, transport requirements.Disinfection and Sterilisation techniques and Sterility control.Blood cultures ? proper taking of samples, culture systéme icl. BacTec, BactAlert. Anearobic Culture. Anaerostat.
Annotation
Provides basic information on general and specialised human medical microbiology, virology, mycology and parasitology. Describes important human pathogens, their characteristic properties, pathogenicity mechanisms of the diseases they induce, and basics of the antibiotic, antifungal, antiviral and antioparasitic therapy of the diseases.
In practical hands-on learning provides important skills for bacteriological investigation of selected samples. The student will learn technics for clinical specimen collection, strategy of microbial identification and anti-microbial therapy.
