Sylabus
* Lectures
General features of metabolism. Bioenergetics.
Digestion, absorption, storage and transport of saccharides.
The citric acid cycle.
Regulation of metabolism of saccharides.
Biomembranes. Composed lipids.
Lipoproteins: Classification, properties and metabolism.
Steroids: Synthesis and metabolism. Steroid hormones. Bile acids.
Conversion of amino acids to biologically active derivatives.
The connective tissue, bone and cartilage. Proteoglycans, collagen and elastin - metabolism.
Saliva.
* Seminars
Biologic oxidation, mitochondrial respiratory chain and oxidative phosphorylation
Stereochemistry of saccharides
Metabolism of saccharides.
Overview of basic methods used in biochemical lab: photometry, chromatography, electrophoresis, blotting.
Lipids: Biosynthesis and degradation of fatty acids
Relationships between metabolism of lipids and saccharides. Starvation
Metabolism of amino acids: Transamination, deamination, synthesis of urea.
Porphyrins and bile pigments
Metabolism of calcium, phosphate, and other elements and vitamins necessary for mineralisation of bone and teeth.
Tooth: Chemical composition and structure
* Practical lessons
Saccharides I: Colored reactions of saccharides. Analysis of unknown saccharide.
Saccharides II: Acidic hydrolysis of starch. Substrate specificity of amylase and sucrase. Polarimetry of glucose and fructose.
Introduction to spectrophotometry: Absorption spectra of colored solutions. Calibration curve, factor calculation.
Saccharides III: Estimation of glycemia. Glucose in urine.
Saliva: Demonstration of selected components
Lipids: Estimation of total cholesterol and triacylglycerols. Demonstration of double bonds in fatty acids. Test for malondialdehyde. Ketone bodies in urine.
Liver tests: Direct and indirect bilirubin. Estimation of aminotransferases.
Bone and teeth: Estimation of calcium and phosphate in serum and urine. Isoenzymes of alkaline phosphatase. Properties of dental hydroxyapatite.
Anotace
Obsahem biochemie a patobiochemie stomatologického směru budou základní poznatky o přeměně látek v organismu ve vztahu k nejdůležitějším a nejčastějším nemocem se kterými se stomatolog při své praxi může setkat (diabetes, poruchy hemokoagulace). Podrobně budou probrány zejména trávicí procesy, obrana organismu proti infekci (zánět) a metabolismus pojiva a tvrdých tkání (zuby, kosti ).
Zvláštní pozornost bude věnována metabolismu vápníku a jeho poruchám a dále biochemii a patobiochemii ústní dutiny (sliny, zubní plak).