Syllabus
Basic properties of bacteria, their virulence factors, pathogenesis of infections. G+ and G- cocci, G+ and G- rods, Mycobacteria, Mycoplasmas, Chlamydiae, Rickettsiae, Spirochets. Basics of medical mycology and parasitology. Desinfection and sterilization, Antimicrobial drugs and strategy of therapy. General and special virology, pathogenesis of viral infections, immune mechanisms of the host, vaccination. HIV, HAV,HBV,HCV,HDV,HEV, Herpesviridae, Neurotropic viruses, Viral diarrhoeas. Respiratory Tract infections, Sexually transmitted infections. Infections with exanthemas and other skin and mucose manifestations. Oral Cavity microbial flora and infection processes. Diagnostic Techniques in medical microbiology.
Practicals 1 (one week, total 26 hours):
Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques - hybridisation, PCR. Horizontál electrophoresis, real-time PCR. Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test. Sample collection techniques and Kits, documentation, transport requirements. Disinfection and Sterilisation techniques and Sterility control. Blood cultures - proper taking of samples, culture systéme icl. BacTec, BactAlert. Anearobic Culture. Anaerostat.
Practicals 2 (one week, total 26 hours):
Students practisize collection of their own samples: throat swab, nose swab, urine and transport them to the laboratory. During the week they follow algorithm of routine bacteriological laboratory work-flow hands-on. Additionaly they obtain coded patiens samples: urine, stool, vaginal swab and blood culture. They investigate each sample making different tests. At the end they have to provide results for each sample: Determined pathogen and its sensitivity to antibiotics Additionaly they perform Immunocard tests for different agents: viral or bacterial. They interpret serological tests and detect genome of EBV or CMV using PCR
Seminars (Attendance Compulsory):
Rational antimicrobial therapy (3 hours)
Prevention and therapy of viral diseases (3 hours)
Annotation
Basic knowledge of microbiology, special dental part of microbiology, virology, mycology and parasitology. Human patogens, their properties, diagnostics, immunization, antibiotics, antibacterial and virostatic therapy, vaccination.
Microbiological apects of microflora of oral cavity, special dental microbiological profile. Methods of treatment.
Practice: methods of sample collection of biological materials, methods of identification, stategy of antibacterial therapy - antibiotic centre, antibiotic police, interpretation of results of examination.