Sylabus
* Lectures:
Mutations and their repair mechanisms
Molecular basis of genetic diseases
Methods of DNA analysis, gene mapping, DNA libraries, functional genomics
DNA diagnostics, direct, indirect, impact on medicine
Gene manipulations, gene therapy, its perspectives
Population Genetics, population equilibrium, factors influencing equilibrium, consanguinity, small populations, medical relevance
Immunogenetics I - genetics of alloantigenic make up of organisms (blood groups, HLA)
Immunogenetics II - immune response genetics
Genetic programming of ontogenesis
Teratogenesis - teratogens, prevention and diagnostics of congenital defects
Oncogenetics I - genetic characteristics of tumor cells, protooncogenes
Oncogenetics II - tumor supressor genes, mutator genes, presymptomatic diagnostics of cancer
Medical genetics, impact on medicine
Gene expression in its normal state and in pathology
* Practical exercises:
Introduction to population genetics
Hardy-Weinberg equilibrium, selection, and mutation
Migration, small populations, genetic drift
Molecular Genetics I. DNA, RNA, proteins, and DNA variability
Molecular Genetics II. PCR, Sanger sequencing, direct DNA diagnostics
Molecular Genetics III. Next-generation sequencing, direct DNA diagnostics continued
Molecular Genetics IV. Indirect DNA diagnostics
Forensic identification of individuals by molecular genetics methods
Immunogenetics I: histocompatibility systems, transplantation principles
Immunogenetics II: Microscopical preparations demonstrating syngenic and alogenic transplantations of skin, kidney and tumor tissues. HLA - disease associations.
Developmental Genetics, Teratogens
Oncogenetics
Principles of the genetic counselling, risk evaluation, indication of preventive measures.
Anotace
Molekulární biologie a genetika. Imunogenetika.
Genetika ontogenese. Onkogenetika.
Mechanismy evoluce. Ekogenetika.
Lékařská genetika.