Sylabus
Introduction to Medical Microbiology, Basic Disciplines. Role in Health Care System. Characteristics of microorganisms- bacteria, viruses, fungi and parasites
Identification Techniques for Microbes
Morphology and Physiology of Bacteria, Disinfection and Sterilisation Technics, Methods of Sterility Control
Interaction of bacteria and the host - Pathogenicity and Virulence,
Antimicrobial agents and antimicrobial therapy.
Review of Medical Bacteriology.Bacterial Genetics
Gram-negative Bacteria, Spirochets and Mycoplasma
Mycobacteria, Chlamydiae, Rickettsiae
Viruses. Taxonomy. pathogenesis and intracellular parasitism in the host cell
Selected DNA Viruses
Prions and prionic infections
Selected RNA Viruses
Medical Mycology
Parasitology
Seminars (Attendance Compulsory):
Diagnostic Techniques in Medical Microbiology
Rational antimicrobial therapy
Practicals:Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques ? hybridisation, PCR. Horizontál electrophoresis, real-time PCR.Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test.Sample collection techniques and Kits, documentation, transport requirements.Disinfection and Sterilisation techniques and Sterility control.Blood cultures ? proper taking of samples, culture systéme icl. BacTec, BactAlert. Anearobic Culture. Anaerostat.
Anotace
Provides basic information on clinical microbiology, virology, and parasitology. Based on the recent classification, reviews the most important bacteria, fungi, and parasites of humans, their features, pathogenesis, diseases.
Reviews microbiological diagnostic techniques, prevention, vaccination, and therapeutic methods. During the practicals, provides the opportunity to test correct sampling, subsequent basic laboratory diagnostic methods, pathogen identification, and antibiotics sensitivity testing, and strategy of ATB therapy.