Syllabus
Lectures:
1. Antibody immune response and regulation. T-independent, T-dependent antigens. Immunology basis of vaccination
2. Immune function of T cells - Th1, Th2, Th17, regulatory T cells. Cytotoxic reactions, NK, NKT cells
3. Mucosal and skin immune reaction. Inflammation and immune reaction
4. Break of the immune tolerance, autoimmunity-mechanisms and risk factors
5. Antiinfection immunity. Prions and the immune system. Primary immunodeficiency - molecular basis
6. Immunopathology reactions of hypersensitivity, Coombs and Gell Type I - IV Practicum I: Immune proteins analysis - immunoglobulins, specific antibodies (against infectious and other antigens), acute phase proteins, paraproteins Methods for detection of autoantibodies - indirect immunofluorescence, ELISA, Western Blott (WB) Complement tests, tests of the complement function (including complement inhibitors) Theoretical principles of: immunodifusion, precipitation, agglutination, immunoelectrophoresis / immunofixation, radio/enzymoimmunoanalysis, turbidimetry and nephelometry. Practical demonstration: typical patterns of autoantibodies detection by indirect immunofluorescence and westernblott Based on case studies Practicum II: Methods for analysis of cell mediated immunity Flow cytometry - principle of the method. Phenotypization of the cells - application in different areas. Analysis of the activation state of leucocytes, thrombocytes and other cells. Functional tests of lymphocytes - cytotoxicity, proliferation, cytokine secretion Functional tests of phagocyting cells - ingestion, metabolic burst of Neu Practical demonstration: analysis of membrane markers of peripheral blood lymphocytes Based on case studies
Annotation
The Course of Module IIA covering more advanced mechanisms of immunity and dominantly abnormal functions of immune system