Syllabus
A) Analysis of nucleic acids:
1) DNA and RNA isolation
2) Measurement of DNA and RNA concentration
3) PCR
4) Methods of mutations detection (RFLP, PCR with allele specific primers)
5) Electrophoresis of DNA and RNA
6) Real-time PCR*
7) Detection of microRNA*
8) DNA sequencing* B) Analysis of proteins:
1) Proteins isolation
2) Measurement of proteins concentration
3) Electrophoresis of proteins (SDS-PAGE)
4) Western blot analysis
5) Fluorescent microscopy
6) ELISA*
7) Flow cytometry* *only theoretical lessons