Syllabus
* Sylabus:
Day 1 Monday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol
Lectures: Introduction to stem cell biology and biomaterials (Jendelová), Safety, laboratory work (Růžička)
Practical course: NA
Day 2 Tuesday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol
Lectures: Embryonic stem cells and induced pluripotent cells, Neural stem cells: their isolation, characterization and use in experiments (Jiráková, Jendelová)
Practical course: seminar - MSC isolation from rat bone marrow (femur, tibia), preparation of primary cultures (video with comment, 15 min), - Implantation of hydrogels into a hemisection model of SCI (video with comment, 30 min), - Balloon compression lesion model of SCI (video with comment, 15min), - Behavioural tests to evaluate motor and sensory function in a rat model of SCI (video with comment, 20 min
Day 3 Wednesday: Institute of Experimental Medicine ASCR. Videnska 1083, Prague 4, Building LA
Lectures : NA
Practical session (Růžička): Work in a flow box, preparation of materials for tissue engineering (nanofibers, hydrogels, hydroxyapatite), seeding of MSCs - Cell culture (media exchange, passaging, cell counting, basic microscopy), Checking of confluence, Passaging, cell counting, Seeding stem cells on materials (10min)
Day 4: Thursday Department of Neuroscience 2.LF UK
Plzeňská 130/221, Prague 5 - Motol
Hematopoetic stem cells and mesenchymal stem cells from bone marrow, adipose tissue and Wharton Jelly and their use in regenerative medicine (Vacková), Cell labeling with iron-oxide nanoparticles and their monitoring by MR imaging. (Jendelová)
Practical session (Růžička) -Microscopy -Analysis of cell survival in spinal cord injury, -Image analysis software - Image J (cell counting, area summation), Axiovision Wizard software, Image analysis - hydrogel properties, cell attachment (10 min), Nanofibers - idea of orientation Hydrogels - surface characteristics, porosity, modifications, Confocal vs. fluorescence microscopy
Day 5, Friday: Department of Neuroscience 2.LF UK Plzeňská 130/221, Prague 5 - Motol
Advance therapy medicinal products (ATMP) in clinical and preclinical studies (Kubinová)
Annotation
Embryonální, fetální, mezenchymální a tkáňově specifické kmenové buňky; teoretické základy, vývoj v oboru.
Praktické aplikace kmenových buněk v terapii závažných onemocnění.