Syllabus
Introduction to tissue engineering
• Tissue structure – overview
• Selected techniques and methods applied in tissue and protein engineering – summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)
• Regenerative medicine and tissue engineering
• Cells in tissue engineering – principle sources, cells isolated from tissues, embryonic and adult stem cells
Tissue engineering
• Cartilage tissue engineering
• Bone tissue engineering
• Skin covers
Nanofibers, their production and safety
• Nanofibers and production methods
• Safety of nanofibers and other nanosystems
• Medical textiles as scaffolds for cell seeding
• Reasons and methods of nanofiber functionalization
Bio-nanotechnology and systems of controlled drug release
• Application of bio-nanotechnology in tissue engineering and regenerative medicine
• Controlled drug delivery, liposomes, their preparation and application
• „Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules
Bio-nanotechnology for artificial tissue production
• Exogenous and endogenous cell sources
• Bio-nanotechnology and mobilization of endogenous cell sources
• Biomechanical properties on native and artificial tissues and their determination
• Quality testing of bio-implants (in vitro and in vivo tests)
Practicals– UCEEB CTU Martin Královič martin_kralovic@labdemo.cz
Univerzitní centrum energeticky efektivních budov ČVUT v Praze
Laboratoř pokročilých biomateriálů
Třinecká 1024 273 43 Buštěhrad
A. Nanofibers and controlled drug delivery (UCEEB CTU)
Task 1: Preparation of nanofibers by electrospinning and forcespinning methods
Task 2: Visualization of nanofiber's morphology (SEM analyses)
Task 3: Demonstration of 3D printing method
Task 4: Evaluation of physico-chemical properties of nanofibres (FTIR analysis)
B. In vitro characterization of cells Institute of Experimental Medicine of the ASCR (IEM ASCR) eva.filova@iem.cas.cz
Institute of Experimental medicine of the ASCR
IBC building
Vídeňská 1083 142 20 Praha 4
Task 1: Cell passaging
Task 2: Cell staining, confocal microscopy – DiOC6 and propidium iodide staining
Task 3: Cells metabolic activity testing – MTS test