Sylabus
* Introduction to tissue engineering
Tissue structure - overview
Selected techniques and methods applied in tissue and protein engineering - summary (lasers, fluorescence, flow cytometry, FACS, electromigration and chromatographic methods, PCR, spectroscopic and spectrometric methods)
Regenerative medicine and tissue engineering
Cells in tissue engineering - principle sources, cells isolated from tissues, embryonic and adult stem cells
* Tissue engineering
Cartilage tissue engineering
Bone tissue engineering
Blood-vessel tissue engineering
Skin covers
* Nanofibers, their production and safety
Nanofibers and production methods
Safety of nanofibers and other nanosystems
Medical textiles as scaffolds for cell seeding
Reasons and methods of nanofiber functionalization
Bio-nanotechnology and systems of controlled drug release
Application of bio-nanotechnology in tissue engineering and regenerative medicine
Controlled drug delivery, liposomes, their preparation and application
„Smart nanofiber/liposome systems“ for controlled delivery of bioactive molecules
* Bio-nanotechnology for artificial tissue production
Exogenous and endogenous cell sources
Bio-nanotechnology and mobilization of endogenous cell sources
Biomechanical properties on native and artificial tissues and their determination
Quality testing of bio-implants (in vitro and in vivo tests)
* Experimental part
* A. Nanofibers and controlled drug delivery (UCEEB CTU)
Task 1: Preparation of nanofibers by electrospinning and forcespinning methods
Task 2: Visualization of nanofiber's morphology (SEM analysis)
Task 3: Evaluation of physico-chemical properties of nanofibers (FTIR)
Task 4: Demonstration of 3D printing method
* B. In vitro characterization of cells (IEM ASCR)
Task 1: Cell passaging
Task 2: Cell staining, confocal microscopy - DiOC6 and propidium iodide staining
Task 3: Cells metabolic activity testing - MTS test
Task 4: Visiting the GMP laboratory for human cell and tissue therapy at IBC ASCR