Sylabus
Winter semester 2020/2021
Lectures:
Introduction, history of clinical microbiology, evolution of microbes, bacterial genetics (viruses, bacteria, parasites, mechanisms of horizontal gene transfer - transformation, transduction, conjugation, specific genetic elements - insertion sequences, transposons, integrons, etc.)
Review of medically important parasites (general parasitology, live cycles, vectors, transmission patterns, diagnostics (Trypanosoma spp., Leishmania spp., Trichomonas vaginalis, Giardia intestinalis, Entamobea histolytica, Plasmodium spp., Toxoplasma gondii, Paragonimus westermani, Schistosoma spp., spp., Taenia spp., Echinococcus spp., Ascaris lumbricoides, Enterobius vermicularis, Sarcoptes scabei)
Review of clinically important G+ bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Streptococcus spp., Enterococcus spp., Staphylococcus spp., Bacillus spp., Corynebacterium spp., etc.)
Review of clinically important G- bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Neisseria spp., Haemophilus spp., Enterobacteriaceae, Vibrio spp., Campylobacter spp., Helicobacter spp., Pseudomonas and other non-fermenting rods, Legionella spp.)
Review of clinically important bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Mycobacterium spp., Nocardia spp., Actinomyces spp., Chlamydia spp., Mycoplasma spp., Rickettsia spp., anaerobic bacteria - review of the most important species, e.g. Clostridium difficile, C. perfringens, C. tetani, C. botulinum, Peptostreptococcus spp., Fusobacterium spp.)
Antibiotics (basic groups of antibiotics, mechanism of action, molecules; beta-lactams, glycopeptides, polymyxins, amynoglykosides, quinolones, sulfonamides, chloramphenicol, etc.)
Mechanisms and epidemiology of antibiotic resistance (mechanisms of bacterial resistence, clonal spread of resistant bacteria, horizontal transfer of genes - mobilisation of genes, mobile genetic elements, integrons, co-resistnace, cross-resistance)
Hospital-acquired infections (definition, examples of the most important pathogens, resistence bacteria as a causative agents of hospital-acquired infections - MRSA, VRE, resistant Gram-negative rods - production of extended-spectrum beta-lactamases, carbapenemases)
Review of viral diseases 1 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; papillomaviruses, polyomaviruses, adenovirus, herpesviruses, poxviruses, picornaviruses, coronaviruses, paramyxoviruses, influenza virus, rhabdoviruses, filoviruses, reoviruses, togaviruses and flaviviruses, togaviruses, bunyaviridae)
Review of viral diseases 2 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; retroviruses - HIV, hepatitis viruses)
Indication, limitations, and interpretation of microbiological diagnostics methods
Practical courses: 5.10 - 9.10 Preanalytical Phase, Normal Microflora 12.10. - 16.10. Laboratory Diagnosis of Mycotic Infections 19.10. - 23.10. Laboratory Diagnosis of Parasitic Infections 26.10. – 30.10. Laboratory Diagnosis of Viral Infections
(28.10. public holiday) 2.11. - 6.11. Laboratory Diagnosis of Skin, Joints and Bones Infections, Anaerobic Bacteria 9.11. - 13.11. Laboratory Diagnosis of Urinary Tract Infections, Laboratory Diagnosis of Sexually Transmitted Diseases 16.11 - 20.11. Test, Laboratory Diagnosis of Gastrointestinal Tract Infections
(17.11. public holiday) 23.11. - 27.11. Laboratory Diagnosis of Respiratory Tract Infections 30.11. - 4.12. Sepses and Bloodstream Infections 7.12. - 11.12. Laboratory Diagnosis of CNS infections, Nosocomial Infections 14.12. - 18.12. Antimicrobial Terapy - Case Reports 4.1. - 8.1. Test, Credits