Syllabus
Lectures
Lecturer: RNDr. Věra Králová, Ph.D.
Human chromosomes, chromosome abnormalities.Mendel´s laws, Morgan´s laws, gene linkage.
Single-gene inheritance.Mitochondrial inheritance.
Inheritance of quantitative traits, multifactorial inheritance, heritability of traits.Immunogenetics.
Genomics. Pharmacogenetics.Recombinant DNA technologies, gene manipulations.
The prevention and treatment of genetic diseases.Population genetics.
Practical courses
Lecturers
Prof. Dr. Emil Rudolf, Ph.D.
Dr. Veronika Skarková, Ph.D.
Practical courses
Meiosis and gene linkagePolytene chromosomes in Drosophila Meiosis in cells of locust
Karyotyping of human cells
Cytogenetic tests and sex chromatin Chromosome classification and identification (banding and newer techniques) Karyotype of human cells Sex chromatin in leukocyte cells
Single-cell inheritance (workshop I) Pedigree analysis
Isolation of DNA Isolation of DNA from human cells cultivated in vitro
Molecular techniques Electrophoresis, blotting, PCR
Tumor biology I Biological characteristics of tumor cells
Tumor biology II Malignant transformation of cells – molecular changes
Biological typing in man (workshop II) DNA diagnostics
Credit Credit test
Annotation
Human karyotype. Structural and numerical aberration of autosomes.
Sexual reproduction, meiosis, abnormalities of gonosomes. X-linked genes, X chromosome inactivation. Genetic manipulations - recombination of DNA, PCR, DNA sequencing.
DNA diagnostics. Production of recombinant molecules.
Gene therapy. Genetics of multicellular organisms, Mendel´s law, Morgan´s law. Clinical genetics - inborn errors of metabolism, prenatal diagnosis, genetic counselling.
Human genetics - pedigree analysis, siblings, genomics. Gene control of immunity, antibodies, monoclonal antibodies.
Immunologic tolerance, transplantation immunity. Population genetics - Hardy-Weinberg law - its application and consequences.