Syllabus
Lectures
Lecturer: Doc. RNDr. Věra Králová, Ph.D.
Human chromosomes, epigenetic mehanisms, chromosome abnormalitiesMendel´s laws, Morgan´s laws, gene linkage
Single-gene inheritanceMitochondrial inheritanceInheritance of quantitative traits, multifactorial inheritance, heritability of traits
ImmunogeneticsPharmacogeneticsMolecular genetics methods
GenomicsRecombinant DNA technologies, gene manipulationsPrevention and treatment of genetic diseases
Introduction into the genetics of tumors
Practical courses
Lecturer: Prof. Dr. Emil Rudolf, Ph.D.
Practical courses
Meiosis and gene linkage Meiosis in gene linkage analysis (workshop I)Meiosis in cells of locust
Cytogenetics, cytogenetic diagnostic methods Outline and principles of cytogenetic methodologyKaryotype of human cellsSex chromatin in leukocyte cells
KaryotypePreparation of karyotype from human cells cultivated in vitro
Monogenic inheritance (workshop II)Pedigree analysis, symbols and rules in select human monogenic traits (physiological and pathological)Examples of mechanisms of inheritance and expression of monogenic traits deviating from Mendel´s rules
Basic molecular biology techniques IIsolation of DNA from human cells (+ e-learning)Testing genotoxicity (mutagenity) and carcinogenityFirst progress test (topics 1-4)
Molecular biology methods in medical diagnostics (workshop III)Processing and analysis of isolated nucleic acidsExamples of diagnostic use of molecular methods
Biological typing in man (workshop IV)Use of molecular methods for identification of people – interactive workshop
Polygenic and multifactorial inheritance (workshop V)Examples of traits ad their inheritance – interactive workshopSecond progress test (topics 5-7)
Tumor biologySelect features of malignant cellsSporadic, familial and hereditary tumors – occurrence, differences
Population genetics (workshop VI)Introductory concepts Third progress test (topics 5-7)Credit
Annotation
Human karyotype. Structural and numerical aberration of autosomes.
Sexual reproduction, meiosis, abnormalities of gonosomes. X-linked genes, X chromosome inactivation. Genetic manipulations - recombination of DNA, PCR, DNA sequencing.
DNA diagnostics. Production of recombinant molecules.
Gene therapy. Genetics of multicellular organisms, Mendel´s law, Morgan?s law.
Clinical genetics - inborn errors of metabolism, prenatal diagnosis, genetic counselling. Human genetics - pedigree analysis, siblings, genomics.
Gene control of immunity, antibodies, monoclonal antibodies. Immunologic tolerance, transplantation immunity.
Population genetics - Hardy-Weinberg law - its application and consequences. Evolution of species, evolution of Homo sapiens.