Sylabus
Lectures
Lecturer: prof. PharmDr. Emil Rudolf, Ph.D.
Introduction, organization of living systems, modelling in biomedicineProperties of eukaryotic cells in multicellular organism
Nucleus- structure and functionDNA synthesis
Biological membranes - structure and function, medical relevance
Cytoskeleton - structure and function, medical relevance
Intracellular compartments- structure and function, medical relevance
Cell death – types, principles, regulation
Cell cycle and reproduction, regulation, signalling
Practical courses
Lecturers
Prof. PharmDr. Emil Rudolf, Ph.D.
Doc. RNDr. Vera Kralová, Ph.D.
Introduction to the course. Information sources in biomedicineRules and requirements of the courseScientific information in biomedicine (types, evaluation)Forms and structure of scientific article, electronic bibliographic databases
Microscopy of living cell - split class (group A)Introduction to light microscopyContrast enhancing microscopies in observation of living cellsFluorescence – e-learning course (group B)
Microscopy of living cell - split class (group B)Introduction to light microscopyContrast enhancing microscopies in observation of living cellsFluorescence – e-learning course (group A)
Cell motility – split class (group A)Cytoplasmic streamingFlagellar movementCiliated motilityCell motility – e-learning course (group B)
Cell motility – split class (group B)Cytoplasmic streamingFlagellar movementCiliated motilityCell motility – e-learning course (group A)
Cell cultures in biomedicineCultivation of cells in vitroIntroduction to the tissue culture laboratoryFirst progress test (topics 1-4)
Cellular membranes – fusion and osmosisOsmosis in plant cellsOsmosis in animal cells
Cellular stressMechanisms, factors and outcomes of cellular stressCultured cells in suspension, vital stainingCell stress and demise in human cellsTests of cytotoxicity in vitro – e-learning courseSecond progress test (topics 6-7)
Cell proliferation and its measurementTechniques of cell cycle and proliferation measurementMitotic and proliferation indexes in cultured cells
Meiosis and gene linkageMeiosis and gene linkage analysis- interactive workshopMeiosis in cells of locust
Biological experimentScientific experiment – effect of a chemical factor on cell viabilityThird progress test (topics 8-10)
Anotace
Definice života - organizace živých soustav, modelování v biomedicíně. Buněčná teorie - buňky prokaryontní a eukaryontní, mikroskopické techniky.
Základní stavební prvky buněk, struktura a funkce nukleových kyselin. Jádro, chromozómy - mikrostruktura, makrostruktura.
Syntéza DNA. Realizace genetické informace, transkripce a posttranskripční modifikace.
Buněčné membrány - stavba a funkce, transport látek přes membrány, fúze biomembrán, princip kompartmentace. Endoplazmatické retikulum - stavba a funkce, Golgiho komplex.
Mitochondrie - stavba a funkce, tvorba ATP, chemiosmotická teorie. Cytoskelet (mikrotubuly, mikrofilamenta, střední filamenta.
Reprodukce buněk (mitóza). Pěstování buněk a tkání in vitro, využití v medicíně.
Principy signalizací u prokaryotních a eukaryotních buněk. Stresové odpovědi - buněčná smrt.