Sylabus
Lectures
Prof. Dr. Jan Krejsek, Ph.D.
Prof. Dr. Ctirad Andrys, Ph.D.
Assoc. Prof. Pavlina Kralickova, MD, Ph.D.
Dr. Eva Mala, Ph.D.
Dr. Ondrej Soucek, Ph.D.
Dr. Vladimira Rezacova, Ph.D.
Dr. Karolina Jankovicova, Ph.D.
Dr. Martina Kolackova, Ph.D.
Lecture 1st Principles of immune system function (Krejsek) genetic background of immune response, epigenetic influences natural barriers, microbiota cells, tissues and organs of immune system immunity as an integral part of protective and damaging inflammatory response 2nd Innate immunity, basic characteristics (Malá)
DAMP, PAMP signals, receptors PRR and their function initiation and development of the inflammatory response humoral mechanisms of innate immunity, interferons, complement system cellular mechanisms of innate immunity, phagocytosis, natural cytotoxicity 3rd Specific immunity (Soucek)
TcR, BcR receptors gene rearrangement clonal selection hypothesis of specific immune response
T cells differentiation
HLA system, processing and presentation of antigenic fragments to T cells polarisation of T cells, Th1, Th2, Th17, Treg subsets of T cells
B cells differentiation, antigen independent differentiation
B cell differentiation after antigenic stimulation, isotypic switching, somatic mutation antibodies, molecular characteristics, dynamics of antibody production immune memory 4th Mucosal and skin immunity (Kolackova) barrier function of mucosal surfaces microbiota, its contribution to mucosal and systemic immunity breast feeding, prebiotics, solid food introduction, probiotics nutrition and immunity, proinflammatory activity of adipose tissue skin immune system skin microbiota 5th Protective inflammation/damaging inflammation (Malá) immune response to viral infections immune response to bacterial infections immune response to fungal and parasitic infections active immunisation, mechanisms of action, adjuvans autoimmunity, development immunopathology, mechanisms of development, hypersensitivity reactions immunopathological diseases, systemic, organ specific modulation of inflammation, biological therapy 6th Immunodeficiencie (Kralickova) inherited primary immunodeficiencies diagnostics, management acquired secondary immunodeficiencies immunopathogenesis treatment 7th Cancer and inflammatory response, immunomodulation (Soucek) protective inflammation is effectively controlling malignant cell proliferation some tumours can escape protective inflammation immunotherapy in oncology immunomodulation, active immunisation
Lectures
Prof. Dr. Jan Krejsek, Ph.D.
Prof. Dr. Ctirad Andrys, Ph.D.
Assoc. Prof. Pavlina Kralickova, MD, Ph.D.
Dr. Ondrej Soucek, Ph.D.
Dr. Vladimira Rezacova, Ph.D.
Dr. Karolina Jankovicova, Ph.D.
Seminars and Practicals 1st Allergic inflammation (Kralickova) pathophysiology of allergic inflammation allergens, components of allergens allergic diseases, asthma, rhinitis, eczema …, clinical presentation diagnosis of allergic diseases, skin tests, allergen specific IgE, basophil activation test in vitro 2nd Prevention and treatment of allergic inflammation and anaphylaxis (Kralickova) elimination of allergen exposure treatment of allergic inflammation immunotherapy by allergen emergency in allergology, therapeutical options angioedemas, hereditary angioedema, diagnostic procedure 3rd Immune deficiencies (Kralickova) primary and secondary immunodeficiencies (including AIDS) clinical presentation diagnosis, immunological testing immunomodulation, immunosubstitution, immunopotentiation 4th Immunopathology, autoimmunity (Jankovicova) immunopathology, initiation, pathophysiological mechanisms, hypersensitivity reactions immunopathogenesis of selective autoimmune diseases (rheumatoid arthritis, systemic lupus, Crohn disease, celiac disease) systemic and organ specific immunopathological diseases autoantibodies and other biomarkers in differential diagnosis of immunopathological diseases 5th Cell mediated immunity (Soucek) hybridoma, CD markers, flow cytometry routine test of cell mediated immunity (T, B and NK cells) tests to determine parameters of phagocytosis and cell proliferation immunophenotyping of blood malignancies 6th Immunological detection of infectious agents (Rezacova) direct evidence of infection indirect evidence of infection detection of specific antibodies as indirect evidence of infection detection of specific cell-mediated immune response as indirect evidence of infection (IGRA tests) immunological diagnosis of B. pertussis, B. burgdorferi, T. pallidum and M. tuberculosis infections clinical interpretation of serological tests
Anotace
Fundamental of Medical Immunology is realized in the forms of lectures, seminars and practicals. The aim is to introduce the complex and modern concept of the function of the immune system as principal integrating body system.
The emphasize is put on the understanding of the role of cellular substrate of immune response, differentiation of immune cells and description of their function. Special emphasize is given to gain the knowledge of the participation of immune system into the both protective and adverse inflammatory response.
Protective inflammatory response against invasion of pathogenic microbes including active immunisation is described. Adverse inflammation is characterized on the molecular and cellular level with the respect to specific characteristic of this type of inflammation in various clinical contexts.
Various attempts how to modulate adverse inflammatory response including administration of biologics is described. The basic principal of the interaction between immune system and cancer is given including the role of cancer environment and abuse of immune response is given.
Mechanism of action of different biological drugs to overcome cancer cell escape from immune surveillance are described. The primary and secondary immunodeficiencies, including HIV infection and AIDS are presented.
The diagnostic procedures and the way how to modulate immune system in immunodeficient patients are given. The basic framework of immunological test used in clinical practice is explained.
The indication and interpretation of these immunological tests is given in clinical context of differential diagnostic procedures.