Sylabus
Lectures
Lecturers Prof. Jaroslav Mokry, M.D., Ph.D. Prof. Stanislav Filip, M.D., Ph.D. Assoc. Prof. Pavel Zak, M.D., Ph.D. Tomas Soukup, M.D., Ph.D. 1 Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research. (Mokry, 3) 2 Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration. (Mokry, 3) 3 Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury. (Mokry, 3) 4 Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation. (Mokry, 1; Filip, 1; Žák, 1) 5 Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors. (Soukup, 3) 6 Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages Orthopaedic applications. (Soukup, 3)
Practical courses and seminars
Lecturers Tomas Soukup, M.D., Ph.D. Assoc. Prof. Jakub Suchanek, M.D., Ph.D.
Practical courses1 Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.2 Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.3 Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.4 Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.5 Visit of tissue culture laboratory. Cell viability. Preparation of cells for flow cytometric analysis.
Anotace
Předmět navazuje na znalosti cytologie. Studenty seznamuje s diskutovanými tématy současnosti, jakými bezpochyby jsou: kmenové buňky, buněčné kultury v biomedicíně, buněčná terapie, regenerativní medicína, tkáňové inženýrství apod.
V praktických cvičeních jsou studenti obeznámeni se základy práce v laboratoři tkáňových kultur a s detekčními metodami.