Sylabus
Lectures
Professor: prof. PharmDr. Emil Rudolf, Ph.D.
Introduction, modelling in biomedicineProperties of eukaryotic cells in multicellular organismsNucleus- structure and function.
DNA synthesis and expression (transcription and translation)
Biological membranes - structure and function, medical relevance
Cytoskeleton - structure and function, medical relevance
Cell cycle and reproduction, regulation, signalling
Cell stress – pathways and responses
Cell death – types, principles, regulation
Practical courses
Instructors:
Prof. Dr. Emil Rudolf, Ph.D.
Assoc prof. Dr. Věra Králová, Ph.D.
Dr. Kateřina Dvořáková
Introduction to the course, information sources, microscopyRules and requirements of the courseScientific information in biomedicine – biology (types, databases)Introduction to light microscopyMock test – 1st lecture
Microscopy of living cells – split class (group A)Group A - Progress test 1Contrast enhancing microscopies in observation of living cellsGroup B - Fluorescence – e-learning course and Progress test 2 (Moodle)
Microscopy of living cells – split class (group B)Group B - Progress test 1Contrast enhancing microscopies in observation of living cellsGroup A - Fluorescence – e-learning course and Progress test 2 (Moodle)
Cell cultures in biomedicineProgress test 3Cultivation of cells in vitroIntroduction to the tissue culture laboratory
Cellular membranes – fusion and osmosisProgress test 4Osmosis in plant and animal cellsCell fusion
Cell motility – split class (group A)Group A - Progress test 5Cytoplasmic streaming, Flagellar and ciliated motilityGroup B - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell motility – split class (group B)Group B - Progress test 5Cytoplasmic streaming, Flagellar and ciliated motilityGroup A - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell proliferation and its measurementProgress test 7Techniques of cell cycle and proliferation measurementMitotic and proliferation indexes in cultured cells
Cellular stress – split class (group A)Group A - Progress test 8Cultured cells in suspension, vital stainingCell stress and demise in human cellsGroup B - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Cellular stress – split class (group B)Group B - Progress test 8Cultured cells in suspension, vital stainingCell stress and demise in human cellsGroup A - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Meiosis and genetic recombinationProgress test 10Meiosis and gene linkage analysis- interactive workshopMeiosis in cells of locust
Anotace
Definice života - organizace živých soustav, modelování v biomedicíně. Buněčná teorie - buňky prokaryontní a eukaryontní, mikroskopické techniky.
Základní stavební prvky buněk, struktura a funkce nukleových kyselin. Jádro, chromozómy - mikrostruktura, makrostruktura.
Syntéza DNA. Realizace genetické informace, transkripce a posttranskripční modifikace.
Buněčné membrány - stavba a funkce, transport látek přes membrány, fúze biomembrán, princip kompartmentace. Endoplazmatické retikulum - stavba a funkce, Golgiho komplex.
Mitochondrie - stavba a funkce, tvorba ATP, chemiosmotická teorie. Cytoskelet (mikrotubuly, mikrofilamenta, střední filamenta.
Reprodukce buněk (mitóza). Pěstování buněk a tkání in vitro, využití v medicíně.
Principy signalizací u prokaryotních a eukaryotních buněk. Stresové odpovědi - buněčná smrt.