Syllabus
Lectures
Professor: prof. PharmDr. Emil Rudolf, Ph.D.
Introduction, modelling in biomedicineProperties of eukaryotic cells in multicellular organismsNucleus- structure and function.
DNA synthesis and expression (transcription and translation)
Biological membranes - structure and function, medical relevance
Cytoskeleton - structure and function, medical relevance
Cell cycle and reproduction, regulation, signalling
Cell stress – pathways and responses
Cell death – types, principles, regulation
Practical courses
Instructors:
Prof. Dr. Emil Rudolf, Ph.D.
Assoc prof. Dr. Věra Králová, Ph.D.
Dr. Kateřina Dvořáková
Introduction to the course, information sources, microscopyRules and requirements of the courseScientific information in biomedicine – biology (types, databases)Introduction to light microscopyMock test – 1st lecture
Microscopy of living cells – split class (group A)Group A - Progress test 1Contrast enhancing microscopies in observation of living cellsGroup B - Fluorescence – e-learning course and Progress test 2 (Moodle)
Microscopy of living cells – split class (group B)Group B - Progress test 1Contrast enhancing microscopies in observation of living cellsGroup A - Fluorescence – e-learning course and Progress test 2 (Moodle)
Cell cultures in biomedicineProgress test 3Cultivation of cells in vitroIntroduction to the tissue culture laboratory
Cellular membranes – fusion and osmosisProgress test 4Osmosis in plant and animal cellsCell fusion
Cell motility – split class (group A)Group A - Progress test 5Cytoplasmic streaming, Flagellar and ciliated motilityGroup B - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell motility – split class (group B)Group B - Progress test 5Cytoplasmic streaming, Flagellar and ciliated motilityGroup A - Cell motility – e-learning course and Progress test 6 (Moodle)
Cell proliferation and its measurementProgress test 7Techniques of cell cycle and proliferation measurementMitotic and proliferation indexes in cultured cells
Cellular stress – split class (group A)Group A - Progress test 8Cultured cells in suspension, vital stainingCell stress and demise in human cellsGroup B - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Cellular stress – split class (group B)Group B - Progress test 8Cultured cells in suspension, vital stainingCell stress and demise in human cellsGroup A - Tests of cytotoxicity in vitro – e-learning course and Progress test 9 (Moodle)
Meiosis and genetic recombinationProgress test 10Meiosis and gene linkage analysis- interactive workshopMeiosis in cells of locust
Annotation
Definition of life, organisation of living systems. Molecules in cells, structure and function of proteins and nucleic acids.
Cell theory, prokaryotic and eukaryotic cells. The cell nucleus, organisation of DNA, chromosomes.
DNA replication and repair. Transcription and translation of genetic information, RNA processing, posttranslational modification.
The cellular membranes - structure and function, transport across biomembranes, cell membrane fusion. The endoplasmic reticulum, the Golgi apparatus.
The mitochondrion, production of ATP. The cytoskeleton.
Regulation of cellular functions - principles of cell signaling.Interaction of cells with the environment. Cell death, necrosis, apoptosis.