Syllabus
Thematic areas of lectures
1. Introduction to phytochemical methods.
2. Methods of extraction and isolation
3. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) I
4. Chromatographic methods (TLC, prep. TLC, HPLC, HPLC/ MS, GC, GC/MS ) II
5. Spectroscopic methods for identification of secondary metabolites (IR, NMR, MS)
6. Overwiev of secondary metabolites Lectures are held every odd Wednesday 8:50 – 10:20 a.m. in the seminary room in the Garden of medicinal plants. Practical classes Preparation of capillary, Pasteur pipete, pen for preparative thin layer chromatography Thin layer chromatography: identification of unknown compound Preparative thin layer chromatography Preparation of alkaloid extract from plant Flash chromatogryphy GC/MS analysis of alkaloid extract and identification of alkaloids GC-FID identification of galanthamine, lycorine and haemanthamine in extracts Preparative HPLC+ HPLC analysis of herbal tea
Annotation
In this subject the students familiarize with the special isolation and analytical procedures, which are involved in the work with plant material for the effective isolation of compounds of different types and for determination of content of secondary metabolites by the modern analytical methods.
In the practical classes the students familiarize with the procedure of processing of row material which leads to the identification of compounds.
The education is directed to creative approach during the isolation of compounds and phytochemical analyses. The subject is intended for applicants who are interested in the diploma thesis with phytochemical theme.