Charles Explorer logo
🇬🇧

Biotechnology

Class at Faculty of Pharmacy in Hradec Králové |
GAF231

Syllabus

Biotechnology.

Basic terms.

History of biotechnology.

Industrial biotechnology.

Biotechnological process.

Industrial microorganisms.

Fermentation equipment, mixing, aeration, sterilization.

Culture media.

Microbial growth and product formation.

Fermentation monitoring and control.

Downstream processing.

Recombinant technology.

Protoplast and cell fusion.

Recombinant DNA technology.

Protein engineering.

Production of recombinant proteins.

Enzyme technology.

Enzymes and cells as biocatalysts.

Immobilized biocatalysts.

Industrial biotransformations.

Plant biotechnology.

Plant tissue culture.

Transgenic plants.

Animal biotechnology.

Animal cell culture.

Hybridoma technology.

Transgenic animals.

Pharmaceuticals produced by means of biotechnological methods.

Small molecules and biopharmaceuticals.

Antibiotics.

Immunosuppressant.

Alkaloids.

Enzymes.

Enzyme inhibitors.

Cytokines.

Monoclonal antibodies.

Fusion proteins.

Vitamins.

Amino acids, dipeptides.

Organic acids.

Oligosaccharides and polysaccharides.

Living cells and organisms.

Vaccines.

Genes.

Annotation

A basic course in biotechnology. The subject describes and explains concepts and theoretical principles underlying the biotechnology-based development and manufacturing of biologically active natural products as well as the other biotechnological applications relevant to the human health.

Special attention is paid to biopharmaceuticals (manufacturing and applications).