Syllabus
Lectures
Model organisms in molecular biology
Structure and organisation of prokaryotic and eukaryotic genomes
DNA replication
Cell cycle, cell death, cell cycle disturbancies
Genetic mutations
Genetic repair, recombination
Structure and types of RNA
Transcription + posttranscriptional modifications of RNA
Genetic code, ribosomes, translation
Posttranslational modifications, protein degradation
Regulation of gene expression in prokaryotes
Regulation of gene expression in and eukaryotes
Isolation of nucleic acids
Overview of enzymes used in experimental molecular biology
Polymerase chain reaction and its modifivations, real-time PCR
Gel electrophoresis
DNA sequencing
Hybridisation, blotting
Molecular cloning, methods of gene transfer
Hereditary diseases
Molecular diagnostics
Introduction to proteomics
Bioinformatics
Annotation
Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function.
Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.