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Molecular Biology and Genetics

Class at Faculty of Pharmacy in Hradec Králové |
GAPS296

Syllabus

Lectures

Model organisms in molecular biology

Structure and organisation of prokaryotic and eukaryotic genomes

DNA replication

Cell cycle, cell death, cell cycle disturbancies

Genetic mutations

Genetic repair, recombination

Structure and types of RNA

Transcription + posttranscriptional modifications of RNA

Genetic code, ribosomes, translation

Posttranslational modifications, protein degradation

Regulation of gene expression in prokaryotes

Regulation of gene expression in and eukaryotes

Isolation of nucleic acids

Overview of enzymes used in experimental molecular biology

Polymerase chain reaction and its modifivations, real-time PCR

Gel electrophoresis

DNA sequencing

Hybridisation, blotting

Molecular cloning, methods of gene transfer

Hereditary diseases

Molecular diagnostics

Introduction to proteomics

Bioinformatics

Annotation

Molecular biology deals with the study of the cellular processes on molecular level. The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function.

Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology) in Medicine and Pharmacy.