Syllabus
Lectures
Aims and specific features of bioanalysis of drugs
Withdrawal and storage of samples of body fluids before analysis
Immunochemical methods of drug monitoring (enzymoimmunoanalysis, radioimmunoanalysis)
Physico-chemical methods (chromatographic, spectral, electroanalytical)
Techniques and procedures of adjustments of samples of body fluids before analysis (Liquid-liquid extraction, SPE, protein precipitation, etc.)
Chromatographic methods with a stress on HPLC
Problems of interference of the biological matrix
Interpretation of results of the found drug levels in body fluids
Therapeutic Drug Monitoring
Analytical toxicology
The use of mass spectrometry in the field of Bioanalysis
Seminars and Practical trainings
Adjustments of samples of blood, plasma, urine before analysis with the use of physico-chemical methods
Liquid-liquid extraction, SPE, protein precipitation, optimization of procedures of isolation of selected drugs from samples of body fluids
Use of TLC as a screening tool in drug poisonings
Use of HPLC in bioanalysis, optimization of chromatographic conditions, selection of the detector, selection of the internal standard, interference of the residues from the biological matrix, verification of the correctness and precision of the elaborated methodology
Bioanalysis of drugs utilizing enzymoimmunoanalysis and radioimmunoanalysis
Therapeutic drug monitoring
Analytical toxicology
The use of mass spectrometry in the field of Bioanalysis of drugs
Annotation
The subject Bioanalysis of Drugs introduces the students the most important methods and techniques, which are widely used in clinical practice to determine drug levels in body fluids with a view to therapeutic drug monitoring, analytical and forensic toxicology, etc.). In clinical practice, there is an increasing demand for university graduates trained in this specialization.
The reason is the fact that all principal questions concerning the fate of the drug in the organism can be answered only by an analytical study. Both theoretical and practical education focuses on physical-chemical and immunochemical methods of drug bioanalysis, problems of the interference of the biological matrix, chromatographic methods with an emphasis on HPLC and mass spectrometry, techniques and procedures of sample preparation prior to the analysis itself and on the most widely employed bianalytical methods.
The students are also introduced to the trends in above-mentioned areas. Some seminars and practical demonstrations take place at ICBD at the Faculty Hospital in Hradec Králové.