Syllabus
Introduction to special methods of instrumental analysis, classification of methods, principles.
Atomic-absorption spectrophotometry, absorbing medium, resonance spectral lines, instrumentation, analytical applications. Atomic-fluorescence spectrometry.
Luminescence analysis, fluorescence, phosphorescence, chemiluminescence, emission and excitation spectra, fluorimeters, spectrofluorimeters, qualitative and quantitative applications.
Turbidimetry and nephelometry
High-frequency spectroscopic methods, nuclear magnetic resonance, NMR spectrum, analytical applications, mass spectrometry, mass spectrum, analytical applications.
Electromigration methods, electrophoretic mobility, principles of separation, instrumentation, analytical use for the separation of biological macromolecules. Capillary zone electrophoresis, capillary gel electrophoresis, capillary isotachophoresis.
Isoelectric focusation, micellar electrokinetic chromatography. Capillary electrochromatography. Instrumentation, applications.
Ion-exchange chromatography. Ion-exchange phenomena, cation and anion exchangers, ion-exchange equilibria, amino acid analyzers, molecular sieves, gel-permeation chromatography.
Pulse voltammetric methods. Difference pulse polarography. Stripping voltammetry.
Automation of analytical procedures, titrators, types of analyzers and their applications.
Flow-injection analysis, sequential injection analysis, automation of analytical procedures, principles, instrumentation, applications.
Annotation
Basic course of special instrumental methods. Development, optimization and validation of bioanalytical methods.