Syllabus
Introduction to the topic, history of topic, various types and characteristics of biomatrix, its sampling and handling.
Sample preparation methods: enzymic hydrolysis with ß-glucuronidase / arylsulfatase, physical and chemical deproteination, filtration, solvent pH adjustment, derivatization, importance of internal standard, various types of the extraction - liquid-liquid extraction (LLE), solid-phase extraction (SPE), solid-phase microextraction (SPME), supercritical fluid extraction (SFE), molecularly imprinted polymers (MIPs).
Utilization of the derivatization in analysis of exogenous compounds in biomaterials, various types of the derivatization reactions and reagents
Preparation of analytical sample in clinical toxicology, special isolation procedures
Bioanalysis of illegal addictive compounds (drug of abuse), specificity in sample preparation
An overview of the instrumental analytical methods employed in analysis of exogenous compounds: methods of the saturation analysis (RIA etc.), HPLC, GC, CZE, importance of the hyphenation (tandem) methods in the identification of chemical structures of phase I and phase II metabolites.
Mass spectrometry in analysis of exogenous compounds in biomaterials
Centrifugation and ultracentrifugation techniques
Sample preparation prior mass spectrometric detection
Chiral compounds, nomenclature, terminology, chiral analysis in biomatrices
Tissue processing, tissue preservation, bank tissue, ethical problems connected with tissue withdrawal
Validation of analytical methods, devices and softwares from ABM point of view.
Accreditation of laboratories, activities in GLP mode, connecting terms.
Annotation
The subject Analysis of exogenous compounds in biological materials (ABM) provides information on the methods of bioanalysis involving both sample preparation and the instrumental analysis (HPLC, CZE, GC using coupling with various detectors). The subject is partly carried out in the laboratories of Faculty Hospital in Hradec Králové.