Annotation
The aim of the course is to present to students the current trends in the analysis of substances of natural origin from the point of view of detection and analytical techniques, and especially from the point of view of effective biologically active substances found in plants, fruits and vegetables, which are most often used in the current directions of bio-pharmacy. These include, in particular, antioxidants, phenolic acids, flavonoids, anthocyans, phytosterols, carotenoids, flavonolignans, alkaloids and others. The aim of the subject is also to clarify the principles of extraction of substances from plant materials using modern extraction techniques such as supercritical fluid extraction and their subsequent separation and identification using modern techniques such as ultra-high performance liquid chromatography (UHPLC) and capillary electrophoresis (CE) in conjunction with mass spectrometry (MS). The subject is also an analysis of mycotoxins, and other contaminants in natural plant materials. An integral part is also the analysis of bioactive substances in food supplements based on plant extracts.
Individual directions in the analysis of substances of natural origin will focus on the following areas:
- Interpretation of analytical data (NMR spectra, mass spectra, IR spectra, Raman spectra) of substances of natural origin;
- high resolution HRMS mass spectrometry in the analysis of natural substances and plant extracts,
- HRMS in the analysis and identification of toxics causing poisoning;
- Use of rapid LC / HRMS methods to diagnose poisoning early;
- use of gas chromatography (GC / MS) in plant extracts analysis
- use of CE/MS in plant extracts analysis
- statistical analysis of data.