Anotace
Practical hands-on training on 3D correlative light and electron microscopy combining confocal fluorescence imaging and scanning electron microscopy with focused ion beam milling (FIB-SEM).
Light, particulary fluorescence, microscopy offers live-cell compatibility and flexibility in labelling by specific molecular probes. In this way, insight into the function of cells at their native state can be obtained. Electron microscopy, on the hand, provides unmatched spatial resolution. Correlative light and electron microscopy (CLEM) combines the unique information from both techniques in a single image.During the course you will try hands-on a complete CLEM workflow from sample preparation to image processing. The hands-on sessions will be conducted using the state-of-the-art equipment available at IMCF, including high end fluorescence confocal microscopes and a scanning electron microscope (SEM) with focused ion beam (FIB) milling.