Syllabus
e-mail: miroslav.kolarik@natur.cuni.cz, tel. 777880129
Rozvrh lekcí pro zimní semestr šk. roku 2016/17
Where?Seminárium B2, Benátská 2, Praha 2
When?Thursday, 12:20-14:40.
Who? Miroslav Kolařík (katedra botaniky Přf UK/MBÚ AVČR)
Milan Gryndler (MBÚ AVČR)
Ondřej Koukol (katedra botaniky Přf UK)
Karel Prášil (katedra botaniky Přf UK)
Jan Holec (Národní Muzeum v Praze) 1. Introduction and organismal level 1.1. History of fungal classification and their position in tree of the tree of live 1.2. Vegetative thalus, dimorphism, polymorphism, pleomorphism 1.3. Sexual reproduction and associated morphological structures 1.4. Asexual reproduction and associated structures, conidiogenesis 1. lesson (Prášil) 6.10. 2. FUNGAL PHYSIOLOGY 2. 1. Introduction, nutrient income, physiology, transport. Fungi and heavy metals. 3. lesson (Gryndler) 13. 10 2. 2. Physiology of growth and reproduction. 4. lesson (Gryndler) 20.10 2.3. Effect of physical and chemical characters on fungi. 2.4. Communication between fungi and with their environment. 5. lesson (Gryndler) 27.10 3. FUNGAL ECOLOGY 3. 1. Decomposition of litter and wood. Soil, excrement, keratinophylic and anthracophylic fungi. Rock fungi. Aquatic fungi. 6. lesson (Koukol) 3.11 3.2 Endophyte and other symbiosis with plants. Trophic interactions with bacteria, protists, invertebrates and vertebrates. Nematophagous fungi and fungi associated with insect. Competition in fungi. 7. lesson (Koukol) 10.11 3.3 Fungi and humans, biotechnology, fungi in silico. Fungi versus technology - contamination and degradation. 8. lesson (Koukol) 17.11 4. BIODIVERSITY OF FUNGI 4.1. Fungal diversity of fungi in Czech R, Europe and worldwide. Methods used in diversity inventories. 4.2. Species numbers in relation to various habitats and geographical regions. 4.3. Factors affecting fungal diversity on various levels. 2. lesson (Holec) 24.11 5. FUNGAL GENETICS 5. 1. Genetics of sexual reproduction. 5. 2. Genetics of asexual reproduction. Parasexual cycle. 9. lesson (Kolařík) 1.12 5.3. Fungal genome. Flow cytometry in mycology. 5.4. Fundamentals of molecular taxonomy, isolation of fungal DNA. Molecular markers used. 10. lesson (Kolařík)8.12 5.5. PCR fingerprinting. Environmental sample analysis. 11. lesson (Kolařík) 15.12 5.6. Genes used in molecular taxonomy and identification. Paralogs. Databases. Hybridization. 12. lesson (Kolařík) 5.1
Annotation
Status of Fungi and their position in the Kingdoms of living nature, phylogeny of Fungi. The cell level: biology of the fungus' cell, the comparative ultrastructure of Fungi, the membrane systems and transport, communication of fungus' cell and environment.
The organismal level: fungal thallus, dimorphism and pleomorphism in fungi, dikaryotic fungi, expression of sexuality, sexual and asexual reproduction, conidiogenesis. Physiology of the nutrition, growth and reproduction.
Genetics of sexual reproduction, genetic analysis of meiospores sets, parasexual hybridization. Molecular taxonomy and DNA based identification.
Genome structure. Moleuclar markers used.
Databases. Analysis of environmental samples.