Syllabus
The 5-days course includes introductory lectures (L) and individual practical works (P).
Day 1: L: yeast taxonomy; yeast morphology; occurrence in nature; biotechnology applications P: yeast colony morphology; yeast cell morphology, staining of the cell components (light microscopy); inoculation; yeast immobilization in alginate beads
Day 2: L: mating; sporulation; cell cycle regulation; budding pattern P: mating and sporulation induction; cdc mutant strains (light microscopy); test of mutagenicity using S. cerevisiae
Day 3: L: yeast cell metabolism P: growth curve; static cultivation; carbohydrate fermentation test; yeast biosensor (copper detection)
Day 4: L: major yeast pathogens (identification,virulence factors,…) P: chromoagar, Candida test; biofilms, adhesion test, invasive growth
Day 5: L: killer phenomenon in yeasts; aging; autophagy P: killer yeast identification; autophagy (fluorescence microscopy)
Annotation
The 5-days course includes introductory lectures and individual practical works. Practical works include following: identification of the yeasts, mating of yeasts, sporulation, immobilization of cells, the production of extra-cellular proteins, killer phenotype, adhesion test, detection of copper level (yeast biosenzor), test of mutagenicity using S. cerevisiae strain.