Annotation
In this three-day practical course, participants will learn basic molecular cloning techniques from in silico cloning strategy to the practical molecular cloning of the selected gene into a plasmid-type expression vector with subsequent verification of the construct using classical molecular biological methods (PCR, restriction endonuclease digestion or sequencing). Then the course participants will try to express the selected protein in a bacterial expression system.
This part of the practical course includes the electroporation of the constructed plasmid into the appropriate bacterial strain and the subsequent induction of the expression system to produce the selected protein, including the subsequent analysis of protein production by PAAGE electrophoresis.