Syllabus
Direct methods - PCR, qRT PCR, sequencing
Indirect methods - imunofluorescence, ELISA
Emphasis will be on good clinical practice. The course will mimic the situation in the clinical laboratory.
Annotation
This practical course will focus on molecular methods of viral detection in clinical specimens including the interpretation of results. The methods will include extraction of nucleic acids from different type of clinical material. The students will analyze the quality and quantity of nucleic acids. They will also detect the selected genes by the polymerase chain reaction (PCR) and/or real time PCR. The specificity of amplicons will be confirmed by sequencing.
For the detection of viral specific antibodies Enzyme-Linked Immunosorbent
Assay (ELISA) and/or indirect immunofluorescence method will be used.
The course will be held in BIOCEV, Vestec from Oct 23 to 27