Syllabus
1. Introduction - Physiology of bacteria Characteristics of bacteria and archea, History of bacteria physiology, Strategy in physiological research and genomic revolution, Model organism - Escherichia coli, Bacteria: laboratory pure cultures and bacterial communities
2. Structure and function of bacterial cell Chemical composition and structure of bacterial cell, DNA, nucleus and genomes of bacteria, Proteins, Flagella, pili and fimbriae, Cases, S-layers, Outer membrane of gram-negative bacteria, Cell wall and periplasmic space, Cytoplasmic membrane, Cytoplasm and cytoplasmic compartments
3. Transport in bacteria Transport in bacteria, lipids, membrane proteins, Ionophores as models Types of transport, diffusion, primary and secondary transport, ABC transporters, phosphotransferase system, precursor and product antiport, iron transport, Native protein conformation, chaperones and chaperonins, Protein transport to CPM, periplasms , OM, excretion of proteins into the environment,
4. Bacterial nutrition, energy, catabolism and anabolism. Bacterial nutrition, Glycolysis and its alternatives, Krebs cycle, Energy production and transformation in bacterial cell, Aerobic respiration
5. Biosynthesis and growth Molecular composition of bacteria, Nitrogen and sulfur assimilation, Biosynthesis of amino acids, nucleotides, lipids, Biosynthesis of cell wall structures, Individual cell growth, cell cycle, Growth of bacterial population, Continuous cultivation
6. Metabolic diversity Anaerobic fermentation, Anaerobic respiration, Chemolitotrophy, Phototrophy.
Annotation
The aim of the lecture is to give the student a comprehensive idea of the structure and function of bacterial and archaea cells, which are the implementation of their genetic program depending on the changing environmental conditions.
Prerequisite of the course is Microbiology MB140P33I.