DNA, genom (anotace genomů), organizace, proteiny vázající genom, replikace - regulace elongace replikace, kolize repl. a transkripce, nukleové kyseliny jako \"počítače\".
2. RNA I
Transkripce: iniciace tnx v detailu-kinetika (např. closed, open complex), malé molekulové effektory (ppGpp, iNTP), elongace, pausing, terminace, prokaryotická \"čepička\", nanoRNA.
3. RNAII
Transkripce, alternativní sigma faktory (sigma K), transkripční faktory,transkriptomické experimenty: microarrays, chip on chip, RNAseq.
4. Regulace genové exprese
Riboswitches, phosphoswitches, ribozymes.
5. Malé RNA
Malé RNA v bakteriích (6S RNA, tmRNA, porovnání s eukaryoty).
6. Adaptace na nepříznivé podmínky
Kaskáda sigma faktorů - kompetence - kanibalismus - sporulace, bistabilita, bet hedging, Deinococcus, systémy toxin-antitoxin.
7. Tvar buňky
Buňka jako celek/tvar - MreB, spirály MinCDE, lokalizace, nanotubes.
8. Regulace translace.
9. Proteiny
Proteomy a další -omy, přístupy, výsledky, modely / databáze.
10. Syntetická biologie - vznik, přístupy; aplikace základního výzkumu
The lecture is based on primary data from original research papers. The lecture illustrates how models of biological processes are derived from experimental data. The aim of the lecture is to stimulate the ability to critically evaluate and interpret experimental results. The lecture expects the knowledge of the basics of molecular biology. However, experimental approaches required for understanding the experiments will be explained. THE FOCUS WILL BE ON LOGICAL THINKING AS OPPOSED TO DATA-MEMORIZING.
","inLanguage":"en"}]}The lecture is based on primary data from original research papers. The lecture illustrates how models of biological processes are derived from experimental data.
The aim of the lecture is to stimulate the ability to critically evaluate and interpret experimental results. The lecture expects the knowledge of the basics of molecular biology.
However, experimental approaches required for understanding the experiments will be explained. THE FOCUS WILL BE ON LOGICAL THINKING AS OPPOSED TO DATA-MEMORIZING.
The lecture is based on primary data from original research papers. The lecture illustrates how models of biological processes are derived from experimental data.
The aim of the lecture is to stimulate the ability to critically evaluate and interpret experimental results. The lecture expects the knowledge of the basics of molecular biology.
However, experimental approaches required for understanding the experiments will be explained. THE FOCUS WILL BE ON LOGICAL THINKING AS OPPOSED TO DATA-MEMORIZING.