1. Introduction of immunologically important organs and tissues at the mouse model, preparation of immune cell populations
2. Culture of these cells, their differentiation and activation3. Histological staining of dry imprints and smear samples4. Fluorescent staining and flow cytometry5. Immunoglobin isolation, protein electrophoresis, western blotting6. ELISA7. Indirect immunofluorescence,microscopy8. NO assay
Aim of this course is the introduction to some general methods used in immunological research by using them actively in the lab. All lectures are given in English.
Attendance at this course assumes a basic knowledge of immunology, graduation of practical courses of Cell Biology and Histology is recommended.