Sylabus
1. Introduction to Transgenesis - Basic principles and strategies, transgenic organisms
2. Molecular genetics - Definition of gene function, mouse genetics and genetic mapping
3. Epigenetics. Imprinting and inactivation of chromosome X
4. Gametogenesis, fertilization, human and mouse stem cells
5. Transgenesis I. BAC transgenesis. Binary transgenic systems. Conditional targeting and homologous recombination. Recombination systems
6. Transgenesis II. Design of vectors for transgenesis. Electroporation and selection. Preparation of chimeras / transfer to germinal lines
7. Reporter mice and lineage tracing
8. Principles of mouse phenotyping. Pivotal mouse models and phenotypes
9. Modeling Human Diseases I. Muscular system
10. Modeling Human Diseases II. Gastrointestinal system
11. Modeling Human Diseases III. Nervous system, neurodegenerative diseases
12. Modeling Human Diseases IV. Cancer
13. The CCP phenotypic platform. Ethical and legal aspects of animal model-based research
Anotace
The dynamic development of transgenic technologies in recent years allows the study of functions of individual genes not only in the context of different cell types but also in the highly complex environment of whole organisms. The mouse is a classical mammalian model whose study has defined entire disciplines such as immunology, developmental and tumor biology, or stem cell biology.
At present, laboratory mouse is the primary model in biological and biomedical research. The lecture provides a basic overview of transgenic models and their application in research.
The principles of Mendelian genetics, epigenetics, gametogenesis and basic procedures for the preparation of transgenic models are explained in detail. The main attention is paid to the use of transgenic mice for modeling human diseases and their phenotyping, leading to the unravelling of thepathophysiological background of the disease.
The ethical and legal aspects of research on transgenic models are also mentioned.