Syllabus
1. Light microscopy (Sádlová, Vojtková, Majer, Bečvář, Jelínková, Hammerbauerová, Vajs)Introduction to the basic theory of light microscopy and handling the light microscope.
Students will prepare native and stained microscopic slides (Giemsa-Romanowski staining, aniline blue and native alizarin staining). The techniques will be demonstrated on protozoa with various taxonomic affiliations and helminths. 2.
Special light and fluorescence microscopy (Sádlová, Vojtková, Majer, Bečvář, Jelínková, Hammerbauerová, Vajs)Students will be introduced to the basics of the theory of contrasts in light microscopy (dark field, phase contrast, DIC, Hoffman contrast), fluorescence and confocal microscopy. They will practically learn the work with dark field, phase contrast and fluorescence microscope. 3.
Electron microscopy (Sacherová, Juračka, Hyliš) Students will be introduced to the theory of electron microscopy and preparation of specimens. They will have the chance to work practically with scanning and transmission electron microscopes and prepare simple specimens.
Annotation
Microscopic techniques is an exercise aimed to introduce students to light, fluorescence and electron microscopy.
The course consists of 3 blocks. The itinerary and all information you can find on Moodle: Přírodovědecká fakulta / ► Sekce biologie / ► Katedra parazitologie / ► MB160C45, with the password "Mikroskop-2023", active from 13th September 2023