Syllabus
1. Introduction, Kinetoplastea I. Basic ultrastructure of Euglenozoa, characteristics of main groups. „Bodonina“: Demonstration of preparations.
2. Kinetoplastea II. Monoxenous kinetoplastids. Morphological forms in the life cycles of trypanosomatids. Demonstration of cultures, dissection of infected hosts.
3. Kinetoplastea III. Patogenic trypanosomes and leishmanias, vector and vertebrate stages, microscopic determination of main representatives.
4. Diplomonadida and Retortamonadida. Ultrastructure, demonstration of main representatives. Giardia - diagnostic, cultivation in vitro, preparation of smears in anaerobic chambers.
5. Praeaxostyla and Parabasalia. Ultrastructure, demonstration of cultures, dissection of infected hosts. Demonstration of pathogenic trichomonads of human and animals and their diagnostic.
6. Pathogenic amphizoic amoebas. Naegleria, Acanthamoeba, Balamuthia. Ultrastructure,microscopic discrimination, cultivation.
7. Human intestinal amoebae. Entamoeba histolytica. Cultivation, laboratory diagnostic. Microscopic diagnostic of intestinal amoebae from training slides.
8. Gregariny and Cryptosporidium. Basic ultrastructure of Apicomplexa and the specific features of gregarins and cryptosporidia. Dissection of infected hosts. Detection of oocysts of cryptosporidium in the native preparations and in the stained smears.
9. Coccidia: Eimeria, Toxoplasma, Sarcocystis, Frenkelia. Life cycles and ultrastructure. Determination of developmental stages in the histological sections, demonstration of oocysts.
10. Microspora. Ultrastructure and life cycles of most important genera. Diagnostics and preparation of slides of selected species from the infected insects and crustaceans. Demonstration of fixed preparations from fish, mammals and human. (Dr. M. Hyliš).
11. Haemosporida and Piroplasmida. Ultrastructure and life cycles. Haemosporina of birds (Haemoproteus. Plasmodium, Leukocytozoon). Identification of Theileria and Babesia on the blood smears and organ prints. Causative agents of human malaria and their microscopic diagnostic.
12. Ciliophora and other parasitic protists. Demonastration of preparations.
Annotation
Advanced laboratory course focused on morphology, ultrastructure and determination of parasitic protists. Parasitic protists will be demonstrated in the form of living cultures, dissections of hosts and mainly in the form on fixed and stained preparations.