Syllabus
The Laboratory Course is scheduled 3 days a week, i.e. on Mondays, Wednesdays and Fridays. The following practical tasks are realised:
1. Anodic stripping voltammetry: determination of Cd cation, optimization of experimental conditions, verification of method´s linearity, determination of limit of quantification, .
2. Conductometry: conductometric titration of various acids (strong, medium, weak), statistical evaluation of results, discussion of titration curves shapes.
3. Automatic potentiometric titration: optimization of conditions for titrimetric determination of divalent manganese by permanganate in the presence of sodium diphosphate.
4. Spectrophotometry: determination of active pharmaceutical compounds in a drug.5. Atomic absorption spectrometry: determination of zinc in drinking water, optimization of experiemental conditions.6. Flow injection analysis: inluence of basic experimental parameters on dispersion of the injected sample.7. Gas chromatography: determination of methanol in fruit spirits.8. High performace liquid chromatography: analysis of caffeine in beverages.9. Capillary electrophoresis: analysis of inorganic anions in drinking water.
Annotation
The following analytical methods are practiced in this course: electrochemical methods - anodic stripping voltammetry, conductometric titrations, and potentiometric titrations; separation methods - gas chromatography, high-performance liquid chromatography, and capillary zone electrophoresis; spectrometric methods - UV/VIS spectrophotometry, atomic absorption spectrometry and flow injection analysis with spectrophotometric detection.
The advanced laboratory course is scheduled 3 days a week, on Mondays, Wednesdays, and Fridays. It is necessary to attend the course all 3 days a week.
After completing the course, the student for these instrumental analytical methods (polarography, anodic stripping voltammetry, conductometry and conductometric titration, potentiometric titration, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- will explain the principle of the analytical methods listed above
- based on the instructions for the task, independently prepares solutions and uses instruments independently (performs measurements)
- interprets measured graphs and curves (polarographic and voltammetric curves, conductometric and potentiometric titration curves, absorption/emission spectra, electropherograms, chromatograms, calibration curves)
- describes and evaluates measured data, draws appropriate conclusions from them
- records the measurement procedure, results, and conclusions in a report