Syllabus
The following tasks are performed:
1. Classical DC polarography: determination of Cd2+ in the sample using the standard addition method2. Conductometry and conductometric titration: determination of hydrochloric acid in the sample3. Karl Fischer titration: determination of water in methanol and flour samples4. Atomic absorption and emission spectrometry: determination of Bi3+ and Cd2+ using F-AAS and K+ using flame photometry5. UV/VIS spectrometry: determination of the dissociation constant of an acid-base indicator6. High-performance liquid chromatography: determination of azo dyes in a mixture using RP-HPLC7. Gas chromatography: qualitative and quantitative analysis of hydrocarbon mixtures Instructions for tasks are available in SIS
Annotation
In this practical training, students will familiarize themselves with modern instrumental analytical methods, which are divided into three main groups: electrochemical (polarography, Karl Fischer titration, and conductometry), separation (gas and liquid chromatography), and spectrometric (visible spectrophotometry, flame photometry, and atomic absorption spectrometry). Before starting each practical task, it is verified whether the student understands the principles of each instrumental method. Emphasis is also placed on the proper processing and statistical evaluation of raw experimental data and the correct presentation of obtained results. The practical training includes ongoing assessment of the knowledge necessary for performing individual tasks, classification of protocols, and laboratory work evaluation.
The practical training session is held at the end of the semester (December) in two-week blocks, and students participate in pairs.
SUCCESSFUL COMPLETION OF THE PRACTICAL TRAINING IS RECOMMENDED AFTER COMPLETING THE COURSE MG431P99 ANALYTICAL METHODS IN GEOCHEMISTRY OR MC230P85 ANALYTICAL CHEMISTRY I+II.
After completing the course, the student for these instrumental analytical methods (polarography, conductometry and conductometric titration, titration according to Karl Fischer, atomic absorption and emission spectrometry, spectrophotometry, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- will explain the principle of the analytical methods listed above
- based on the instructions for the task, independently prepares solutions and uses instruments (performs measurements)
- interprets measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, chromatograms, calibration curves)
- describes and evaluates measured data, draws appropriate conclusions from them
- records the measurement procedure, results, and conclusions in a report