Syllabus
Following exercises are done:1. Classical DC polarography2. Karl Fischer titrations (KF)3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator4. Determination of Bi3+ and Cd2+ ions with AAS and K+ ion using Flame photometry5. Gas chromatography (GC) - Qualitative and quantitative analysis6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection7. Conductometry and conductometric titration8. Capillary zone electrophoresis (CZE)
Annotation
Students become familiar with conventional instrumental techniques of chemical analysis in this practical laboratory course. Taught analytical methods are divided into 3 basic groups: electrochemical, separation and spectrometric methods. The understanding of demonstrated principles of individual instrumental methods is emphasized. Students are led to handle with attained raw experimental data, with their statistical evaluation and also presentation of calculated results. These laboratory exercises are organized in two weeks sessions while students cooperate in small groups. Throughout the course, strict compliance with GLP and safety regulations is required. Students should be able to work with common analytical instruments independently after finishing this course. The integral part of classification is continuous control of knowledge which is necessary to perform each task, classification of protocols and assessment of laboratory work.
After completing the course, the student for these instrumental analytical methods (polarography, conductometry and conductometric titration, titration according to Karl Fischer, atomic absorption and emission spectrometry, spectrophotometry, capillary electrophoresis, gas chromatography with flame ionization detection, liquid chromatography with UV/VIS detection):
- will explain the principle of the analytical methods listed above
- based on the instructions for the task, independently prepares solutions and uses instruments independently (performs measurements)
- interprets measured graphs and curves (polarographic curves, conductometric and potentiometric titration curves, absorption/emission spectra, electropherograms, chromatograms, calibration curves)
- describes and evaluates measured data, draws appropriate conclusions from them
- records the measurement procedure, results, and conclusions in a report