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Analytical chemistry II

Class at Faculty of Science |


* Continuation of the lecture C230P01 - Analytical chemistry I. 12. Absolute and comparative analytical methods. Calibration methods (calibration curve method, standard addition method). 13. Basic statistical evaluation of data (accuracy and precision, mean, standard deviation, confidence interval, valid numbers) 14. Electroanalytical methods based on measurement of potential. Equilibrium potentiometry (electrochemical cell, electrodes). Direct potentiometry (measurement of pH, determination of other ions using ISE, selectivity coefficient, gas sensors based on ISE). Indirect potentiometry, potentiometric titrations. 15. Electroanalytical methods based on measurement of current I. Voltammetry and polarography (polarization curve, scheme of electrochemical cell, electrodes, dc, ac and pulse voltammetry. Voltammetric stripping analysys. Amperometry (amperometric detectors). 16. Electroanalytical methods based on measurement of current II. Electrogravimetry and coulometry (potentiostatic and galvanostatic methods, coulometric titrations, colometric analyzers). Conductometry (conductometric analyzers and detectors). 17. Optical methods (the nature of absorption and emission of radiation by atoms and molecules). Atomic and molecular absorption and emission/fluorescence spectra, their utilization in analytical chemistry. Molecular absorption spectrometry in uv/vis range of radiation. Atomic absorption spectrometry. 18. Automatic chemical analysers (FIA, CFA). Nonspectral optical methods (refractometry, polarimetry, nephelometry). 19. Chromatographic methods (fundamentals, classification of chromatographic methods).

Planar chromatography. Column chromatography (general aspects, parameters obtained from chromatogram, separation efficiency, resolution, optimization of separation process). Application of chromatographic methods in quantitative and qualitative analysis (hyphenated methods, GC-MS, LC-MS). 20. Gas chromatography (columns, detectors). Liquid chromatography (selection of mobile phase, classification of LC based on difference in stationary phase - partition, affinity, size-exclusion, ion-exchange chromatography). 21. Electromigration methods. Separation in electric field (zone and capillary electrophoresis). Example of separation - aminoacids and peptides.

* Seminar's Syllabus: 1. Acid-base equilibria and pH calculations. Acid-base titrations and stochiometry. 2. Redox equilibria, titrations and stochiometry. 3. Basic principles and calculations in separation methods. 4. Statistics of experimental analytical results.


Calibration in instrumental methods. Electrochemical methods: methods based on measuring of potential (equilibrium potentiometry, potentiometric titrations), current (voltammetry and polarography, coulometry, electrogravimetry) and conductivity (conductometry and dielectrometry). Optical methods: methods without exchange of energy (refractometry, polarimetry, nephelometry); absorption spectral methods (molecular absorption and atomic absorption spectroscopy); emission spectral methods (molecular fluorescence spectroscopy, atomic emission spectroscopy); analytical methods in flowing liquids with optical detection; spectral methods for determination of pollutants in the air. Chromatographic methods: column chromatography (gas and liquid), planar (thin-layer) chromatography. Electromigration methods.

In the scope of the Seminar training in basic calculations used in analytical chemistry (gravimetry; precipitation, complexation, acid-base and redox equilibria) is realized.