Syllabus
1. Polarography: influence of oxygen, construction of a calibration curve, standard addition method, logarithmic analysis of a polarographic curve, characterization of investigated analytes).
2. Conductimetry: conductimetric titrations of different acids, statistical evaluation of results, discussion of shapes of titration curves.
3. Automatic potentiometric titration: optimization of conditions for titrimetric determination of divalent manganese by permanganate in the presence of potassium diphosphate.
4. UV/VIS spectrophotometry: determination of the dissociation constant of a coloured indicator.
5. Flame photometry: optimization of conditions for the determination of sodium and potassium.
6. Atomic absorption spectrophotometry: determination of zinc in tape water.
7. Flow injection analysis: the influence of different parameters on sample dispersion.
8. Gas chromatography: qualitative and quantitative analysis of a mixture of hydrocarbons.
9. Liquid chromatography: HPLC analysis of a mixture of azodyes.
10. Separation on ion exchangers: determination of break-through curve of strong katex.
11. Caplillary electrophoresis: qualitative and quantitative analysis of a mixture of nitro compounds.
12. IR spectrometry: structural analysis and multicomponent analysis.
Annotation
Theoretical training for laboratory course. Topics: polarography, voltammetry, conductimetric titrations, potentiometric titrations, gas chromatography, high performance liquid chromatography, ion exchange separation, capillary zone electrophoresis, UV/VIS spectrophotometry, infrared spectrophotometry, atomic absorption spectrophotometry, flow injection analysis.