Syllabus
Volume measuring, samples weighing
Solutions, dissolution, extraction
Chemical composition of biological material
Interaction of electromagnetic radiation with a matter
Spectrophotometry
Chromatography pH and buffers
Protein assay
Isolation of plant DNA
Reversible and irreversible precipitation of proteins
Annotation
The aim of the practical course is to familiarize students with the basic laboratory techniques used in the biochemical laboratory. Groundwork in weighing, work with automatical pipetts, preparation of solutions, measurement of pH, UV/VIS spectrophotometry, chromatography, centrifugation, organisation of the work in the biochemical laboratory.