Příprava roztoků a spektrofotometrie
Precipitace proteinů
pH a pufry
Stanovení celkové hmotnostní koncentrace bílkovin
Volume measuring, samples weighing
Solutions, UV-spectrophotometry
Reversible and irreversible precipitation of proteins
pH and buffers
Protein assay
Volume measuring, samples weighing Solutions, UV-spectrophotometryReversible and irreversible precipitation of proteins pH and buffers Protein assay
The aim of the practical course is to familiarize students with the basic laboratory techniques used in the biochemical laboratory. Groundwork in weighing, work with automatical pipetts, preparation of solutions, UV/VIS spectrophotometry, chromatography, organisation of the work in the biochemical laboratory.