Syllabus
Tasks:
INF/PYM - Basics of bioinformatics and Visualization of biomacromolecules in PyMol software
IEX - Separation of selected proteins by ion exchange chromatography on FPLC
IMAC & WB - Isolation, purification, electrotransfer and immunodetection of recombinant protein (e.g. TEV protease or green fluorescent protein)
X - protein crystallization and methods of biomacromolecules' structure determination
PCR - Polymerase chain reaction of DNA
LIT - Training in work with scientific literature
The course is finished by delivering a short report from one given lab task in a form of short scientific writing.
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The course was innovated within the project ESF for University II at Charles University, reg. No .: CZ.02.2.69/0.0/0.0/18_056/0013322
Annotation
The aim of this practical course is to introduce students to both common and modern instrumental biochemical methods. Besides commonly used techniques, such as extraction of compounds from natural material, UV/VIS absorption spectrophotometry, spectrofluorimetry, chromatographic techniques (HPLC, FPLC, ion exchange, gel permeation and affinity chromatography), electromigration methods (agarose electrophoresis, SDS-PAGE, Western blot - electroblotting with immunodetection), and PCR amplification of DNA, as well as basic bioinformatic training, practical utilisation of biochemical information sources, work with scientific literature and evaluation of experimental results. The course is finished by introduction to principles of text structuring and formal requirements relevant to scientific literature (i.e. bachelor thesis or scientific publications) and subsequent writing of a final report for one given practical task in a form of small scientific work, its timely submission and oral discussion.
The practical course runs on two days in one week, i.e. both on Mondays and Thursdays. The task schedule is available upon registration in Moodle and will be created after the first introductory meeting at the beginning of the course where students pass and sign the safety training and also divide into working groups (two people) - the personal attendance at this meeting is mandatory!
Theoretical introduction to all used techniques, supervision of the exercises and discussion of the obtained results is provided in English to all Erasmus students. The written final report is accepted in English.