Syllabus
1. Gel chromatography of proteins (separation, relative molecular mass determination) 2.
SDS - polyacrylamide electrophoresis (determination of relative molecular mass of proteins) 3. Practical enzymology - kinetic characteristics, Michaelis constants, maximal reaction rate, pH optimum, activation and inhibition of enzymes, electrophoretic separation of isoenzymes and detection of the enzyme activity in gel
Annotation
Practical course in biochemistry is provided by Daniel Kavan, Veronika Hýsková, Josef Chmelík, Kateřina Bělonožníková, Růžena Lišková and Jan Fiala.
The students are dealing with basic biochemical methods. Gel filtration is used for protein purification from a mixture or for relative molecular mass determination. The students determine the relative molecular mass of proteins by SDS polyacrylamide gel electrophoresis. Important part of the practical course is dealing with enzymes, determination of Michaelis constant, maximal reaction rate, pH optimum, activation and inhibition, electrophoretic separation of isoenzymes and their detection in gel.