Syllabus
MEASUREMENT IN CHEMISTRY (analog and digital measurement, signal and noise, reduction of noise and drift, FFT and deconvolution) METHODS TO STUDY THE SIZE AND SHAPE OF MOLECULES (Colligative methods, elastic light scattering, viscosimetry, analytical ultracentrifugation, flow birefringence, dielectric dispersion) METHODS TO STUDY CHEMICAL COMPOSITION AND STRUCTURE (amino acid analysis and protein sequencing, sequencing of polynucleotides, PCR and related techniques, synthesis of peptides, proteins oligonucleotides, chemical modification and immobilization of proteins, mass spectrometry, and hyphenated techniques) DIFFRACTION STRUCTURAL ANALYSIS (basic concepts in crystallography, roentgen, neutron, and electron diffraction) INTERACTION OF ELECTROMAGNETIC WAVES WITH MATTER (physical aspects of spectral measurements, signal and noise, Fourier, Hadamard, multichannel spectrometry, atomic emission spectra, AAS, electronic absorption spectra,derivative and difference spectra UV-VIS, infrared absorption spectroscopy, Raman spectra, resonance and surface-enhanced Raman scattering, spectrofluorimetry, time-resolved fluorescence techniques, bioluminescence a chemiluminescence, opaque materials, reflectance, photoacoustic spectra, chiroptical methods, polarimetry, ORD, CD, nuclear magnetic resonance, pulse, multidimensional NMR, EPR, Mössbauer spectra) STUDY OF CHEMICAL REACTIONS AND EQUILIBRIA (principles of chemical kinetics, time scale, fast reaction kinetics, continuous and stopped-flow methods, relaxation methods, calorimetric methods and thermal analysis, DSC, ligand binding to macromolecules, equilibrium dialysis)
NOTICE: this subject is related to previous subject Metody biochemie MC250P09A, and the competen knowledge of explained methods, principes of techniques an applications are supposed in the subject Metody studia biomolekul - MC250P09B.
Annotation
Physico-chemical techniques in the biochemical laboratory (fundamental theory, instrumentations, applications). Sources of error, signal-to-noise ratio and methods for its improvement. Interaction of electromagnetic waves with matter and potential of spectral methods. Rotational and vibrational (infrared, Raman) spectroscopy, resonance spectroscopy (NMR and EPR spectra, magnetic susceptibility. Mösssbauer spectra). Electronic emission, absorption, luminiscence. Optical activity, CD and ORD spectra. Optoacoustic spectrometry, sonography, and other imaging methods. Size and shape of molecules (elastic light scattering, analytical ultracentrifuge, viscosity, colligative methods, zeta potential, SAXS). Difraction analysis (X-ray difraction). Chemical structure and compositions of biopolymers (mass spectrometry), sequencing and synthesis of proteins, nucleic acids, PCR and related methods of molecular biology. Thermal analysis, calorimetry. Kinetics of biochemical reactions, fermentors, fast kinetic techniques (stopped-flow, relaxation techniques, time-resolved spectral methods), analysis of biopolymer interaction with small molecules.
Lectures - in Czech
This lecture is a loose continuation of MC250O09A (Metody biochemie) and the students are requested to make themselves familiar with that lecture.