Syllabus
Isolation of proteins: different types of separation techniques. Methods used for characterization of proteins; sources of heterogeneity of protein samples, determination of proteins, Structure of proteins: determination of tertiary structure. Chemical modification of proteins, modification in vivo. Affinity techniques in separation of proteins and in characterization of binding properties of proteins.
References:
Biochemistry: R.H. Garrett, C.M. Grisham, Harcourt Brace College Publishers, Fort WORTH, 1995
Proteins: Structure and Molecular Properties: T.E. Creighton, W.H. Freeman 1992
Protein Purification: Principle and Practice, Springer- Verlag 1982
Prof. M. Tichá a H. Ryšlavá
Annotation
Proteins, method of their isolation and characterization, primary structure; affinity technique in the study of binding properties of proteins.
Lectures - in Czech -